XAFS AND CRYSTALLOGRAPHIC STUDIES OF AN AZURIN AND A BLUE COPPER NITRITE REDUCTASE FROM A DENITRIFYING BACTERIUM. S. Samar Hasnain1,2, Fraser Dodd1,2, Richard Strange1, Gunter Grossmann1, Lorreta Murphy1, Zelda Abraham3, Robert Eady3, Bary Smith3, 1Molecular Biophysics Group, Daresbury Laboratory, Warrington WA4 4AD, 2School of Applied Sciences, De Montfort University, Leicester LE1 9bH, 3John Innes Centre, Norwich Science Park, Norwich, UK
Results from a high resolution (~1.7Å) crystallographic studies of oxidised and reduced azurin crystals are compared to the XAFS studies of this new azurin.
Crystal structure study of the first blue copper nitrite reductase will be reported. Results from substrate and ligand binding studies will be presented and the chemical (structural) changes associated with such binding will be discussed in terms of reaction mechanism.
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