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new method
- To: sa_scat@nist.gov
- Subject: new method
- From: vladimir vavilin <viv@erols.com>
- Date: Mon, 20 Jan 1997 07:08:58 -0500
- CC: john.barnes@nist.gov
- Organization: VLAD&DIMA
Dear Colleagues: I am posting this message to get your assistance or advice. I would love to continue the study of plasma lipoproteins by small-angle X-ray scattering ( SAXS )and complete the development of new noninvasive screening method to detect a coronary atherosclerosis in its earliest stages. During the last several years I have been researching on the plasma lipoproteins from human blood by SAXS. As a result, the rapid (within 1 h) technique to determine the fraction distribution of plasma lipoproteins has been developed. A new effective compound like NaBr for "X-ray visualizing" the lipid cores of lipoprotein particles into the complex lipid-protein mixture of human plasma has been found. In contrast to conventional procedures for quantitative determination of plasma lipoprotein fractions, the new method allows one to "fractionate" the total plasma lipoproteins into three major classes such as high density, low density, and very low density lipoproteins without a preliminary separation of human plasma into its constituents. The findings demonstrate the diagnostic application of SAXS in atherosclerosis. At the present time I am living in the United States and looking for the opportunities for applying SAXS as the analytical instrument for coronary atherosclerosis susceptibility express-diagnosis (see attachment). I hope my proposal will find your interest. I am looking forward to receiving your advice and assistance. Thanks in advance. Sincerely, Vladimir Vavilin, Ph.D. 108 Enchanted Hills Rd., Apt.104 Phone: 410-581-1023 Owings Mills, MD 21117 E-mail: viv@erols.com NEW METHOD FOR DETECTING A HUMAN SUSCEPTIBILITY TO ATHEROSCLEROSIS Coronary heart disease is the most frequent cause of death in men over age 35 and women over age 65 in the United States. For example, in 1987 estimated 514,000 Americans were killed by this disease and over 700,000 others were admitted to hospitals because of heart attacks. Coronary heart disease results from the gradual development of atherosclerosis in the coronary arteries. Therefore, it is very important to predict the human susceptibility to coronary atherosclerosis in its earliest stages. High density lipoproteins (HDL) and low density lipoproteins (LDL) fulfilling central functions in the cholesterol transport process by the blood circulation are known to play the key roles in the atherosclerotic pathogenesis. It is also known that high concentrations of LDL as well as low concentrations of HDL in the human bloodstream are associated with the accelerated progression of the coronary atherosclerosis. Hence it follows an important conclusion that it is necessary to measure the absolute concentrations of these two major lipoprotein classes for diagnosing the susceptibility to atherosclerosis. In this connection new method to diagnose the susceptibility to atherosclerosis in humans have been developed. The devised method is based on the analysis of experimental data obtained by means of small-angle X-ray scattering technique which enables one to determine a particle size distribution of lipid cores of major plasma lipoproteins (HDL, LDL and very low density lipoproteins) and evaluated an index of susceptibility to atherosclerosis (LDL/HDL ratio). This method has the considerable advantages in comparison with the conventional procedures for the quantitative determination of the human plasma lipoprotein fractions. It has following advantages: just several drops (0.1mL) of a human plasma need for one analysis that lasts only 60 min and it does not demand a preliminary separation of a human plasma into its constituents. The latter it is very important because the human plasma lipoproteins are labile structures and therefore the isolated lipoproteins can be differed from the native lipoproteins after separation and storage procedures. In conclusion one should note that the proposed method allows one to create quite new analytical instrument for early express-diagnosis of the human susceptibility to coronary atherosclerosis and carry on a mass health screening of the American people for detecting the individuals with high risk of coronary heart disease.
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