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Workshop on small-angle x-ray scattering/diffraction in biology
- Subject: Workshop on small-angle x-ray scattering/diffraction in biology
- From: tsuruta@SLAC.Stanford.EDU
- Date: Wed, 30 Sep 1998 14:56:23 -0700 (PDT)
************************************************************************ Workshop on Small-angle X-ray Scattering/Diffraction in Biology October 21, 1998. Stanford Synchrotron Radiation Laboratory Stanford Linear Accelerator Center Please check out http://www-ssrl.slac.stanford.edu/ssrl25/index.html for registration information. The workshop is held in association with the 25th SSRL users' conference, October 19-20. ************************************************************************ This workshop is designed to give an overview of solution x-ray scattering and low-angle single-crystal diffraction in structural molecular biology. It consists of lectures on recent developments in the field, software demonstrations and hands-on sessions on data collection. Topics in solution scattering include: determining low-resolution structures, modeling quaternary structure, complementing atomic-resolution structures, time-resolved studies and use of x-ray scattering amplitudes in electron microscopy and diffraction. Hands-on sessions and software demonstrations are intended for scientists who are new to the technique. SSRL has developed an instrument which makes it practical to obtain accurately measured single crystal diffraction data in the range of 10-500 A, where the contribution of the molecular envelope is much greater than at higher resolution. The diffraction data in this resolution range are thus critical in phase extension and density averaging. The data have the potential of visualizing parts of structure that are otherwise averaged out to very weak density. Technical issues in dealing with very low resolution data will be discussed. --------------------------------------------------------------------- Preliminary program 9:00 Introduction on small-angle scattering/diffraction - Hiro Tsuruta (SSRL) 9:20 Computational techniques for solution scattering data interpretation - Dmitri Svergun (European Molecular Biology Laboratory) 10:00 Coordinated use of solution x-ray scattering and electron cryomicroscopy of macromolecules - Wah Chiu (Baylor College of Medicine) 10:30 coffee break 10:45 Imaging viral capsids and RNA with low angle solution and single crystal diffraction data. - John E. Johnson (The Scripps Research Institute) 11:15 Modeling viruses and processing data with icosahedral harmonics. - Peter Doerschuk (Purdue University) 11:45 The use of low resolution data in imaging the nucleic acids in icosahedral viruses. - Vijay S. Reddy (The Scripps Research Institute) 12:15 lunch break 13:15 (Phase extension and density averaging using low-res. diffraction data in crystallographic studies on DNA helicase) - Christpher Putnam (The Scripps Research Institute) 13:45 Measurements and analysis of time resolved SAXS for protein folding - Sebastian Doniach (Stanford University) 14:15 A computer system for solution scattering data analysis. [computer software demonstrations] - Dmitri Svergun (EMBL) 15:00 coffee break 15:15 Data collection practice on Beam Line 4-2 [hands-on sessions] 1) solution scattering 2) low-angle single crystal diffraction
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