Event Name

Dilemmas in structural biology: selection and integration of methods

Start Date 14th Feb 2016
End Date 17th Feb 2016
Description

Topics include:

  • Sample preparation
  • Biophysical & biochemical characterization
  • 3D structure determination
  • Structure-based ligand design
  • Structural biology data management

Target Group & Selection process

The workshop targets mainly PhD students and postdoctoral researchers who are currently working in the structural biology field and have already some experience in working with macromolecules. Experienced researchers will also be considered but priority will be given to younger scientists. A total of 20 participants will be accepted in the workshop. The applicants will be expected to send a CV (max. 2 pages), a motivation letter (max. 1 page) explaining why they wish to participate and what they expect from the course and a reference letter. Biostruct-X and local organizers will be responsible for the selection process to ensure that equivalent criteria will be applied.

Benefits

Participants that have been accepted to the course are offered to send protein samples for characterization and crystallization to the Sample Preparation and Characterization (SPC) facility at the EMBL in Hamburg. The samples must be relatively pure (>90 % according to SDS-PAGE). The SPC will offer a preliminary sparse matrix screen that can be customized to the particular sample, as well as complementary characterization by thermal shift assay, dynamic light scattering and mass spectrometry. For more information, please visit the SPC website: http://www.embl-hamburg.de/services/spc/.
Participants are also offered to bring their own protein *(around 60 μl or even less around 10 μl at exceptional cases) and set up their a “Random” Microseeding experiment using the Douglas Instruments protein crystallization robot installed at NHRF under the guidance of the manufacturers (http://www.douglas.co.uk). They should also bring along crystals if any available, to make the seed stock screens. If the condition that the protein crystallizes is known, it is also recommended that participants bring their crystallization buffer as well.

Host

The event will be hosted by NHRF, coordinator of Instruct-EL, the national distributed research infrastructure that functions as a hub for structural biology in South East European area and Cyprus as part of a broader national RI.

More information will be available on NHRF website: http://www.eie.gr/index-en.html

Location Athens
Greece
Contact Dr Evangelia D. Chrysina
echrysina@eie.gr
URL http://www.biostruct-x.eu/content/dilemmas-structural-biology-selection-and-integration-methods
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Category Workshops
Topics Biological macromolecules