Event Name

12th Greta Pifat Mrzljak International School of Biophysics

Start Date 27th Sep 2014
End Date 6th Oct 2014

2014 Topics

Biomacromolecular complexes and assemblies (protein-RNA/DNA complexes, e.g., viruses, ribosomes; quaternary protein structures; DNA, proteins, polyelectrolytes). The lecturers will describe related problems and discuss approaches to solutions, e.g.:

  • spectroscopies (NMR, EPR, FTIR, Raman, MS…)
  • microscopies (AFM, fluorescence techniques, tweezers…)
  • diffraction methods
  • computational solutions, modeling and simulations


  • Nenad Ban, ETH, Zürich, Switzerland: Protein structure & function; X-ray diffraction
    • Ribosomes and their functional complexes
    • Beyond the prokaryotic ribosome
  • Jasna Brujic, NYU, New York, USA: Soft matter, statistical physics; single molecule experiments-AFM
    • Emulsions and packing
    • Single-molecule AFM studies
  • Mario Cindrić, Ruđer Bošković Institute
    • Mass Spectrometry for protein sequencing
  • Yves Engelborghs, KU Leuven, Belgium: Fluorescence microscopy and spectroscopy
    • Time resolved fluorescence spectroscopy of proteins
    • Fluorescence Correlation Spectroscopy : an elegant way to study molecular interactions.
  • Helmut Grubmueller (COST lecturer), MPI-BPC, Göttingen, Germany: Theoretical and Computational Biophysics
    • Forces and Conformational Dynamics in Biomolecular Nanomachines 1,2
  • Jané Kondev, Brandeis University, USA
    • Chromosome Folding in Cells
    • Transcriptional Regulation
  • Pierre-Emmanuel Milhiet, Centre de Biochimie Structurale, Montpellier, France: High-performance AFM, membrane proteins imaging
    • Near field microscopies in structural biology
    • AFM imaging in the field of biological membranes
    • New developments in Bio-AFM imaging
  • Lennart Nilsson, Karolinska Institutet, Sweden: Molecular modeling, nucleic acids, peptides and their interactions
  • Chris Oostenbrink, UNRLF, Vienna, Austria: Structure and dynamics of biomolecules; modeling
    • Ensembles and sampling, leading to molecular dynamics simulations
    • Structure refinement using molecular dynamics simulations (NMR observables)
    • Calculation of free energies from molecular simulation
  • Frances Separovic, University of Melbourne, Australia: NMR and Structural Studies of Membrane-Active Peptides
    • Solid-state NMR of membrane-active peptides
    • Membrane interactions of antimicrobial and amyloid peptides
  • Ana-Sunčana Smith, Ruđer Bošković Institute & Uni Erlangen-Nürnberg Germany: Membranes and vesicles, cell adhesion
    • Membranes, from self assembly to rafts 1,2,3
  • Holger Stark, MPI-BPC, Göttingen, Germany: Protein structure& function; cryoEM
    • Introduction into single particle cryo-EM
    • High-resolution structure determination of macromolecular complexes by cryo-EM
  • Heinz-Jürgen Steinhoff (COST lecturer), University of Osnabrück, Germany: Macromolecular Structure, EPR
    • Principles of site-directed spin labeling EPR
    • Site-directed spin labeling EPR of biomacromolecules,  complexes and assemblies
  • Antonio Šiber, Institute of physics, Zagreb, Croatia: Physics of viruses
    • Physics of viruses: electrostatics, elasticity and DNA condensation in viruses 1,2,3
  • Iva Tolić, Ruđer Bošković Institute & MPI-CBG, Dresden, Germany & RBI, Zagreb: Physics of cells, fluorescence-microscopy
    • Microtubules and motor proteins 1, 2
  • Anthony Watts, Department of Biochemistry, Oxford, UK: Protein structure& function; solid state NMR
    • Principles of solid state NMR for the study of biomolecules
    • Solid state NMR for structural studies of large integral membrane proteins
    • Receptor dynamics and structure in membranes resolved using solid state NMR
  • Bojan Žagrović, MFPL, Vienna, Austria: Computational Biophysics of Macromolecules
    • More dynamic than we think?  On conformational averaging in structural biology 1, 2
    • Protein-RNA interactions and the origin of the genetic code

Program Committee

Location Primošten
URL http://soft.ifs.hr/school/

Category Schools
Topics Biological macromolecules | Computing | Microscopy | X-ray spectroscopy