Event Name

Cryo Electron Microscopy and 3D Image Processing of Macromolecular Assemblies and Cellular Tomography (CEM3DIP)

Start Date 2nd Jul 2016
End Date 13th Jul 2016

The GIAN-CEM3DIP practical course will cover :

  • 3D Electron Microscopy: History and Basic Principles
  • Biological sample and specimen preparation methods for TEM
  • TEM- principles, image formation , particle detection, Fourier Transform, Convolution, CTF, PSF, Resolution and FSC
  • Image processing, Point group symmetries, Classification, Orientation and Heterogenity of particles
  • Getting the first model using Conical Tilt and Projection Matching
  • Single particle CryoEM and 3D reconstruction
  • Challenges of in-situ structural biology
  • Principles and applications of Cryo Tomography. Cellular Tomography and Subtomogram averaging.
  • Refinement of atomic models against CryoEM maps, Flexible fitting, Validation, Assessment and Data deposition.
  • Hybrid methods: Protein Crystallography, Computational methods and CryoEM
Location Thiruvananthapuram
Contact Dr Ramanathan Natesh
URL http://workshop.iisertvm.ac.in/cem3dip/

Category Training courses
Topics Biological macromolecules | Cryo electron microscopy