Event Name

From Genes to Atomic Structures: an Introduction to Synchrotron-Based Structural Biology

Start Date 23rd Apr 2012
End Date 27th Apr 2012

Brief Description:

The last decade has seen an exponential growth in the results obtained by macromolecular crystallography, both in terms of number of structures and their complexity. This growth is having a significant impact on molecular and cellular biology, as more and more macromolecular structures have been instrumental in elucidating biological functions. 

The objective of this introductory workshop is to expose the participants to the methodologies used to solve macromolecular structures, using single crystal X-ray diffraction techniques. Structural biology is an interdisciplinary research area, requiring expertises from both the life sciences and the physical sciences: the school aims to bridge the gap between physics and biology by providing a comprehensive overview of the field, starting from the gene to obtain the atomic structure. A short overview of other structural biology techniques will also be presented. The educational program will be based both on lectures and practical sessions. 

The school is part of a joint initiative between Elettra, IAEA and SESAME, the Middle East synchrotron radiation facility in Jordan.

Main Topics:

  • Bioinformatics analysis to identify structural targets and optimise constructs design
  • Molecular biology and cloning techniques
  • Protein expression and purification
  • Crystallisation
  • Data collection & reduction
  • Structure determination
  • Model building, refinement and model validation
  • Small Angle X-ray Scattering (SAXS)
  • An overview of other structural biology techniques

Location Trieste
URL http://cdsagenda5.ictp.trieste.it/full_display.php?ida=a11160

Category IUCr sponsored meetings | Schools | Workshops
Topics Biological macromolecules | Crystal growth | Data analysis | Small-angle scattering | Synchrotron radiation