There has been some discussion about coordinate frames in relation to PDB SCALE transformations. I would like to emphasize the importance of presenting coordinates in the orthogonal frame chosen by the depositor, which may not necessarily be the default orthogonal coordinate frame. There does not appear to be anything in the mmCIF dictionary that requires that the default frame be used. In discussing residue numbering, the dictionary says: In all cases, the scheme used here must match the scheme used in the publication that describes the structure. By analogy, one should allow the coordinates to be in the same frame as used in the publication. It is not uncommon for a publication to have discussion of symmetry, present transformations, etc. all based on a particular (not necessarily default) choice of coordinate frame. In addition, PDB entries carry information in REMARK and other records that is directly related to the choice of coordinate frame; it would be impossible to convert automatically and correctly between PDB and mmCIF if it were mandatory that the coordinates be transformed into the default coordinate frame when presented in mmCIF. Frances C. Bernstein