IUCr journals

Reconstruction of a yeast cell from X-ray diffraction data

Acta Cryst. (2006). A62, 248–261 [doi.org/10.1107/S0108767306016515]

[Yeast cell]Reconstructed yeast cell image (1 micron scale bar). Hue represents the phase, and brightness the amplitude, of the X-ray exit wave.
Diffraction microscopy (also called coherent diffractive imaging) extends traditional crystallography to the study of non-periodic objects. In this article we present a two-dimensional reconstruction of the image of an unstained yeast cell from its soft X-ray diffraction pattern. We place particular emphasis on algorithmic strategies that deliver reproducible results in the presence of noise and missing data. We also present a method to determine the effective resolution of images reconstructed by this technique.

P. Thibault, V. Elser, C. Jacobsen, D. Shapiro and D. Sayre