IUCr journals

Crystal structure of Mycobacterium tuberculosis mtFabD, a malonyl-CoA:ACP transacylase (MCAT)

Acta Cryst. (2007). F63, 831-835 [doi:10.1107/S1744309107042455]

Mycolic acids - very long α-alkyl, β-hydroxylated fatty acids - are essential and characteristic components of the cell envelope of Mycobacterium tuberculosis, and are synthesised by the joint action of the type I and II fatty acid synthase (FAS) systems. The 3.0 Å crystal structure of M. tuberculosis FabD, which catalyses acyl transfer from coenzyme A to acyl carrier protein of FAS-II, was phased based on anomalous scattering of five ordered Ni2+ ions bound to the His6-affinity tag of the recombinant enzyme.

H. Ghadbane, A.K. Brown, L. Kremer, G.S. Besra and K. Fütterer