High-pressure small-angle neutron scattering studies of glucose isomerase conformation in solution

J. Appl. Cryst. (2009). 42, 461–468 (doi.org/10.1107/S0021889809007456)

The glucose/xylose isomerase from Streptomyces rubiginosus was studied by means of small-angle neutron scattering (SANS) in the pressure range 0.1–150 MPa. Since this relatively large (172 kDa), almost spherical protein is not sensitive to pressure and temperature changes in a broad range, it was selected as a very good candidate for a secondary standard in SANS experiments. Correction of the intensity I(q) resulting from intermolecular interaction described by the structure factor S(q) is also discussed.

E. Banachowicz, M. Kozak, A. Patkowski, G. Meier and J. Kohlbrecher