IUCr journals

Geobacillus stearothermophilus 6-phosphogluconate dehydrogenase complexed with 6-phosphogluconate

Acta Cryst. (2009). F65, 450–454 (doi.org/10.1107/S1744309109012767)

[Phosphogluconate complex] Secondary and domain structure of the Gs6PGDH dimer. The N-terminal domains are blue, the central domains yellow, the C-terminal domains red. The ligand 6PG is shown as van der Waals spheres coloured C black, O red and P orange.
The structure of Geobacillus stearothermophilus 6-phosphogluconate dehydrogenase (Gs6PGDH) complexed with 6-phosphogluconate is reported. 6PGDH is a highly conserved enzyme with conservation most pronounced in the N- and C-terminal domains, which contribute to the active site, compared with the central domain, which is involved in dimerization. 6PGDH is a potential drug target for the parasite Trypanosoma brucei, which causes African sleeping sickness. The T. brucei enzyme is unsuited for ligand studies but the straightforward crystallization means that Gs6PGDH provides a surrogate model for inhibitor design.

S. Cameron, V.P. Martini, J. Iulek and W.N. Hunter