TIME-RESOLVED PROTEIN DATA COLLECTION SYSTEM WITH LARGE IMAGING PLATE. K.Sakabe, N.Kamiya¹, N. Watanabe², S. Adachi¹, K. Sasaki³, S.Ikemizu⁴, T.Higashi⁵, & N. Sakabe⁴, Dept. of Chem. ,Nagoya Univ., Chikusa, Nagoya, 464 Japan, ¹RIKEN, Hirosawa 2- 1, Wako, Saitama, 351 -01 Japan, ²PF, KEK, Tsukuba 305 Japan, ³College of Medical Technology, Nagoya Univ. Higashi, Nagoya, 461, ⁴Institute of Applied Biochem.,Univ. of Tsukuba, Tsukuba ,Ibaraki 305 Japan, ⁵Rigaku Corporation Matsubara, Akishima, Tokyo 196 Japan

We have developed a data collection system which can fit for both time resolved Laue and LOT, and name it as time-resolved camera system. Typical nature of this camera for time-resolved Laue is that imaging plate cassette with 800x800mm ditector area can be moved quickly while rotating fast shutter is chopping the X-ray to get m-see order of time resolution spots whose images are aligned along horizontal direction. Chopping is better than the streaks as following reasons; 1. Easy to get accurate integrate intensity data. 2. Back ground can be reduced extensively. 3 To reduce the dose of X-ray to the crystal and to reduce the crystal damage. The evaluation of this system has been done by crystal of co-amino acid aminotransferase whose space group I222 and cell dimensions are a=137.9, b=124.7, and c=61.5A The normal Laue data collected from 42 frams with 400x800mm. The recovery is 62% within 2Å resolution. The R (I) is 8.4%. Time resolved Laue data was collected from three shots at 2mm interval. The R factor(I) between three spots is 0.07 for 3580 reflection in a frame which corresponds to 75% of single spots exposure in the same condition. Time-resolved expriment using LOT has been done by this system switching to as the Weissenberg camera In the case of tetragonal Iysozyme using flow cell, independent data up to 1.8Å resolution has been collected within 15 min with two frames. Rmerge(I) is 0.045.