Fourth international SPHIRE workshop

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Event Name

Fourth international SPHIRE workshop

Start Date 11th Jun 2018
End Date 13th Jun 2018
Description All stages of the SPHIRE workflow will be covered, with special focus on the:

- optimisation of cryoEM datasets for high-resolution refinement and reproducible analysis of the data

incl. :
  • CTF estimation (CTER) with error assessment
  • robust deep learning particle picking (crYOLO)
  • rapid Stable 2D clustering (ISAC2)
  • Reproducible Initial model generation (RVIPER)
  • automated ML 3D Refinement (MERIDIEN)
  • 3D Variablility analysis
  • Reproducible Focused 3D Clustering (SORT3D) with statistical analysis of variance (ANOVA)

In addition to the practical sessions, short lectures will be given by SPHIRE developers focusing on crucial steps of the processing, possible pitfalls, as well as the theoretical background behind SPHIRE. Registered participants will have the opportunity to present posters on their research work and are also strongly encouraged to bring their own data, since there will be ample time to interact with the instructors and process their own project with SPHIRE during dedicated hands-on sessions.
The course is aimed at graduate students, faculty, and staff, that currently work in or plan to enter the 3DEM field and wish to use SPHIRE to process cryo-EM single particle data at their home laboratories for their own research projects.

Location Dortmund
Contact Christos Gatsogiannis

Category Workshops
Topics Cryo electron microscopy