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10 citations found for Burmeister, W.P.

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Acta Cryst. (1996). A52, C218
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Radiation damage, as can occur during data collections from cryo-cooled protein crystals, has been shown to cause the breakage of disulfide bonds and the decarboxylation of carboxylic acids. Tyrosine and methionine residues are also affected.


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The Vaccinia virus D4/A20 complex is the DNA polymerase cofactor. The heterodimer interface has been analysed using three new crystal structures of the complex in which key residues forming a cation-[pi] interaction have been mutated.

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For a structural genomics project on Epstein-Barr virus, 23 proteins were cloned into E. coli for protein expression. The structures of four have been solved by X-ray crystallography.

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The crystal structure of the complex between the trimeric human adenovirus B serotype 3 fibre knob and desmoglein fragments composed of cadherin domains EC2 and EC3 containing three calcium-binding sites has been determined at 4.5 Å resolution and confirmed by the anomalous signal from terbium ions, which replaced the calcium ions.

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The ESRF undulator beamline ID14 `Quadriga' is dedicated to monochromatic macromolecular crystallography. Using two undulators with 23 mm and 42 mm periods and a minimum gap of 16 mm installed on a high-[beta] section, it will provide high-brilliance X-ray beams at around 13.5 keV, as well as a wide tuneability between 6.8 and 40 keV. Based on the Troika concept, this beamline has four simultaneously operating experimental stations: three side stations, EH1, EH2 and EH3, using thin diamond crystals, and an end station, EH4, with a fast-scan double-crystal monochromator. Station EH3 has a [kappa]-diffractometer, and an off-line Weissenberg camera with a large 80 × 80 cm active area combined with a 2048 × 2048 CCD detector. During data collection the image plates are placed and removed by a robot located inside the hutch using a cassette system. After data collection the image plates are scanned with an off-line drum scanner. Station EH4 is designed for MAD applications, including Xe K-edge anomalous experiments, and is equipped with a 2048 × 2048 CCD detector on a pseudo 2[theta] arm. A common graphical user interface and a database will be available to cover all aspects of data collection, including strategy optimization. First results on the performance of the optics elements and initial crystallographic results are presented.

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