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A low-energy positron beam is a unique probe of materials. In high-energy electron and positron storage rings it is possible to generate intense synchrotron radiation with a photon energy of 1-3 MeV by installing a high-field (8-10 T) superconducting wiggler. High-energy photons are converted to low-energy positrons by using a suitable target-moderator system. For an 8 GeV electron storage ring at a beam current of 100 mA, final yields are estimated to be about 108-1010 slow-e+ s-1 or larger depending on the moderation efficiency, with the size of the positron source 101-102 cm2. In the present work a wiggler magnetic system of 10 T is proposed. The main parameters of the superconducting wiggler are presented.

Pig heart carbonyl reductase has been crystallized in the presence of NADPH. Diffraction data have been collected using synchrotron radiation.

Crystallization and preliminary X-ray crystallographic analysis of rabbit L-gulonate 3-dehydrogenase
The preliminary X-ray crystallographic study of rabbit L-gulonate 3-dehydrogenase is described.

The crystal structure of monkey dimeric dihydrodiol dehydrogenase complexed with the inhibitor isoascorbic acid has been determined at 2.59 Å resolution.

Crystals of AKR1B14 were grown from buffered polyethylene glycol solutions and diffracted to 1.86 Å resolution.

The structure of AKR1C21 holoenzyme was determined at 1.8 Å resolution. A model describing the interaction between AKR1C21 and steroid substrates is proposed that explains the bifunctionality of the enzyme.


Structure of the G225P/G226P mutant of mouse 3(17)
-hydroxysteroid dehydrogenase (AKR1C21) ternary complex is reported at 2.1 Å resolution.
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The structure of the Y224D mutant of mouse 3(17)
-hydroxysteroid dehydrogenase revealed that the mutation resulted in a change in the conformation of the flexible loop B. This loop is a unique feature of the active-site architecture of the wild type and is formed by the side chains of Tyr224 and Trp227.
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Construction of three RF stations in the storage ring of SPring-8 has been completed. The design concept concentrates on avoiding a coupled-bunch instability which limits the stored current or makes the synchrotron radiation beam unstable. The cavity is bell-shaped to reduce the coupling impedance of the higher-order modes. The cavity dimensions are trimmed systematically to distribute the higher-order mode frequencies. Each cavity has two movable tuners. The temperature of the cavity cooling water is controlled within 0.02 K and the water flow is kept constant. The construction and commissioning of the SPring-8 storage ring RF system is reported.

Hexagonal crystals of dimeric dihydrodiol dehydrogenase from monkey were grown from buffered ammonium phosphate solutions. This is the first crystallization report of a dimeric dihydrodiol dehydrogenase.

Orthorhombic crystals of human L-xylulose reductase were grown from buffered polyethylene glycol solutions and diffract to 2.1 Å resolution.

Orthorhombic crystals of mouse 3(17)
-hydroxysteroid dehydrogenase were obtained from buffered polyethylene glycol solutions. The crystals diffracted to a resolution of 1.8 Å at the Swiss Light Source beamline X06SA.
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Absolute calibration of a soft X-ray spectrograph has been performed using a white beam of synchrotron radiation. The calibrated spectrograph was a flat-field grazing-incidence spectrograph with an X-ray CCD detector for X-ray laser research. Absolute sensitivity of the spectrograph system can be obtained from transmitted spectra using filters made of several different materials, each providing an absorption-edge wavelength standard. The absolute sensitivity determined in this work shows nearly the same behaviour with wavelength as that in another calibration experiment using a laser-produced plasma as an X-ray source.

Two novel high-temperature reactors for in situ XAS measurements in fluorescence are presented.

A Suzuki coupling reaction produced the title compound, C44H66N2O2, which consists of a photoisomerizable azobenzene unit coupled to a biphenyl group and a long alkyl chain. The molecule has a trans configuration with respect to the azo (–N=N–) group, and the long alkyl chain adopts a well ordered zigzag arrangement.


The crystal structure of proliferating cell nuclear antigen (PCNA) bound to a peptide carrying an AlkB homologue 2 PCNA-interacting motif consensus sequence, RFLVK, was determined. The phenylalanine and leucine residues of the peptide, plus a preceding hydrophobic residue, are involved in interactions with PCNA, providing a structural basis for regulation of the PCNA interaction.

The crystallization and initial X-ray diffraction of REV7 in complex with REV3 is reported.
