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Cabbage histidinol dehydrogenase has been crystallized in five different crystal forms. These results and initial attempts to find heavy-atom derivatives are described.

Two novel high-temperature reactors for in situ XAS measurements in fluorescence are presented.

An automated large-scale protein-crystallization and monitoring system for high-throughput protein structural analyses has been developed. Many protein crystals have been obtained using the system.

Crustacean hyperglycaemic hormone from the kuruma prawn M. japonicus was crystallized by the sitting-drop vapour-diffusion method in its weakly active precursor form which has an extra glycine residue at the C-terminus. The crystals belonged to the orthorhombic space group P2₁2₁2₁ and diffracted to 1.95 Å resolution.

Crystals are described of the conserved domain IV of E. coli 4.5S RNA, which together with Ffh protein constitutes the bacterial signal recognition particle (SRP) and, by independently binding to elongation factor G (EF-G), also plays an essential role in ribosomal translocation.

An erratum to Acta Cryst. (2000), D56, 1033-1037 .

The structure of the spliceosomal U4 snRNP core domain has been re-refined following molecular replacement using the minimal U1 snRNP as a search model. RNA-omit maps show that the U4 Sm-site nucleotides AAUUUUU are bound to the seven Sm proteins SmF-SmE-SmG-SmD₃-SmB-SmD₁-SmD₂ in the same manner as the U1 Sm-site nucleotides AAUUUGU except at the U-to-G substitution in SmD₁.

A device is described that is useful for the recovery and re-exposure of frozen crystals and for data collection from frozen crystals at different orientations.

The human oncoprotein SET/TAF-1 has been crystallized by the sitting-drop method. A complete diffraction data set was collected to 2.8 Å resolution using synchrotron radiation.