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226 citations found for Shimizu,

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An artificial protein with three complete sequence repeats was created and the structure was determined by X-ray crystallography. The structure showed threefold symmetry even though there is an amino- and carboxy-terminal. The artificial protein with threefold symmetry may be useful as a scaffold to capture small materials with C3 symmetry.


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The performance of a Fresnel-type compound refractive lens for focusing cold neutrons was evaluated. The lens consists of 44 pieces of compound Fresnel-type refractive lenses made of MgF2 single crystals. The intensity profile of the diffracted neutrons measured over the wavelengths of 9-14 Å was compared with the results of numerical simulations. Good neutron transmission of 0.85 for 9 Å neutrons and a focal length of 3.4 m for 16 Å neutrons were achieved.

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The catalytic mechanism and the evolutionary characteristics of thioredoxin derived from the extreme halophile H. salinarum NRC-1 were elucidated by X-ray crystallographic analysis, circular-dichroism analysis and enzymatic assays.

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The tertiary structure of a [beta]-lactamase derived from the halobacterium Chromohalobacter sp. 560 (HaBLA) was determined by X-ray crystallography. Three unique Sr2+-binding sites and one Cs+-binding site were discovered in the HaBLA molecule.

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Acta Cryst. (2005). A61, c191
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Monoclinic crystals of human peptidylarginine deiminase V have been obtained. Native data have been processed at 2.7 Å resolution using the SPring-8 synchrotron-radiation source.

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Crystals of a complex between granulocyte colony-stimulating factor and its soluble receptor have been prepared by the vapour-diffusion method. Two different crystal forms in space groups P41212 and I4122 have been produced using different crystallization conditions.


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A human kynurenine aminotransferase II homologue from P. horikoshii OT3 has been overproduced in E. coli, purified, and characterized. Crystals of this protein have been obtained and analyzed by X-ray diffraction.

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Undecaprenyl diphosphate synthase, a Z-type prenyltransferase, from M. luteus has been crystallized. The crystals diffract to at least 2.2 Å resolution and belong to the monoclinic space group C2.

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The structures of thallium(I) complexes with two different mesityl-substituted ligands, i.e. tris(3-mesityl-5-methylpyrazol-1-yl)hydroborate and bis(3-mesityl-5-methylpyrazol-1-yl)(5-mesityl-3-methylpyrazol-1-yl)hydroborate, are compared. The shape of the former thallium(I) complex can be considered as a vase and the latter as a baseball glove.

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Acta Cryst. (2014). A70, C1127
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The use of predictable coordination geometries and the development of new ligands has allowed supramolecular chemists to design a plethora of new materials. Among these are metal-organic frameworks (MOFs), which are composed of ligands coordinating to metal atoms or clusters to generate a framework with potential porosity. MOFs exemplify supramolecular design strategy as their extended structure and tunable properties allow them to be applied for various applications.1 To date, many MOFs utilize carboxylates as the coordinating group since they have well studied coordination geometries and thus predictable framework topologies. Though there are examples of carboxylate-based MOFs possessing water stability, most do not possess this key feature, hindering their application in industrial settings. Phosphonate monoesters (PMEs) have been investigated as a means to impart water stability to a MOF by kinetically shielding the linker-metal bond with the ester moiety.2 Unfortunately, phosphonate monoesters have relatively unexplored coordination geometries, with most studies focusing on chlodronic acid and its derivatives, which do not typically form porous materials. In an attempt to establish building units based on PMEs, 1,4-benzenediphosphonate monoester ligands have been synthesized, coordinated to Cu(II), and characterized. It was found that while the methyl and ethyl analogues form similar 3-D structures with poor water stability,3 the isopropyl analogue forms a layered material possessing water stability. The isopropyl analogue contains chains of Cu-PME, with the isopropyl esters lying directly above and below the Cu atoms, kinetically shielding this bond from water. This water stable building unit was predicted to generate a porous framework with non-linear ligands. To test this hypothesis, 1,3,5-benzenetriphosphonate monoisopropyl ester was synthesized and coordinated to Cu(II). Unfortunately, no single crystal of sufficient quality has been produced, though a predicted and refined structure matches well to various characterization techniques. As predicted, this material is porous and does not degrade in harsh humid conditions (353K and 90% relative humidity).


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Crystals of RhoGDI complexed with the FERM domain of radixin were obtained and belong to the space group P21212, with unit-cell parameters a = 130.9, b = 151.2, c = 71.2 Å. Diffraction data to a resolution of 2.9 Å have been obtained using synchrotron radiation.

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Crystals of the complex between the FERM domain of radixin and the cytoplasmic tail of ICAM-2 were obtained. The binary complex crystals were found to belong to the space group P3121 or P3221, with unit-cell parameters a = b = 100.44, c = 99.49 Å, and diffract to 2.6 Å resolution.

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