Cryo Electron Microscopy and 3D Image Processing of Macromolecular Assemblies and Cellular Tomography (CEM3DIP)

The GIAN-CEM3DIP practical course will cover :

• 3D Electron Microscopy: History and Basic Principles
• Biological sample and specimen preparation methods for TEM
• TEM- principles, image formation , particle detection, Fourier Transform, Convolution, CTF, PSF, Resolution and FSC
• Image processing, Point group symmetries, Classification, Orientation and Heterogenity of particles
• Getting the first model using Conical Tilt and Projection Matching
• Single particle CryoEM and 3D reconstruction
• Challenges of in-situ structural biology
• Principles and applications of Cryo Tomography. Cellular Tomography and Subtomogram averaging.
• Refinement of atomic models against CryoEM maps, Flexible fitting, Validation, Assessment and Data deposition.
• Hybrid methods: Protein Crystallography, Computational methods and CryoEM