Advanced Work­shop on Cryo-Electron Tomog­ra­phy

May 6â€"7, 2017: Optional Pre-Course
May 8â€"12, 2017: Work­shop

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Con­tent Pre-Course

(Optional for expe­ri­enced par­tic­i­pants)

• Intro­duc­tion to elec­tron tomog­ra­phy and Seri­alEM
• Linux
• Data pro­cess­ing with IMOD and eTomo

Con­tent Work­shop

• Immer­sion freez­ing for cryo-electron tomog­ra­phy
• High pres­sure freez­ing and CEMO­VIS
• Cryo-CLEM
• Low-dose data col­lec­tion with Seri­alEM
• Direct elec­tron detec­tors
• Cryo-STEM tomog­ra­phy
• Pro­cess­ing of low-dose tilt series with IMOD
• Mod­el­ling and inter­pre­ta­tion of cryo-electron tomog­ra­phy data
• Subto­mo­gram aver­ag­ing with PEET

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