Engineering recombinant proteins for structural and functional studies

The programme will address the limiting step in structural studies - the production of
pure and homogenous proteins. Talks and discussions at this event will explore strategies
to optimize membrane and soluble proteins for crystallization, cryo-EM and NMR studies.
Discussions will include construct optimization, novel thermostability assays,
crystallization tools and `tricks of the trade' to enable structure determination.

This event is particlarly directed toward early-stage graduate students in structural biology.
However, anyone interested in generating soluble proteins for a variety of applications,
such as enzymatic assays and antibody production, would be welcome to attend.
Manufacturers of instruments and techniques to assay purity and homogeneity
will be available for hands-on demonstrations.

The list of invited speakers are experienced in the structure determination of
membrane proteins, cytosolic/secreted proteins, and macromolecular signaling complexes:

Edmund Kunji, Cambridge
Daniel Panne, Leicester
Maria Sasi Conte, King?s College
Naomi Chayen, Imperal College
Laura Itzhaki, Cambridge
Chris Tate, Cambridge

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