Electron Crystallography School - 3D Electron Diffraction/MicroED Bridging Small Molecule and Macromolecular Crystallography

School on 3D electron diffraction/MicroED techniques and how to apply them for structure determination of nano/microcrystals too small for X-ray diffraction. The topics will cover inorganic compounds, pharmaceutics and proteins. It includes both lectures and practical sessions.

Topics:

• The development of various 3D electron diffraction/MicroED techniques â€" a historical perspective
• Introduction to transmission electron microscopy and electron diffraction
• 3D electron diffraction/MicroED (SAED, PED, EDT) and space group determinationÂ
• Sample preparation for both materials and life sciencesÂ
• 3D ED/MicroED Data collection  Â
• Radiation damage  Â
• 3D ED Data Processing and data merging including practical demo labs
• Methods for phasing diffraction data â€" small molecules and macromolecules
• Practical demo labs for structure solution and refinement with 3D ED/MicroED data â€" two groups for small molecules and macromolecules, respectively
• Dynamical refinement
• New developments in 3D electron crystallography