LINXS Workshop- Structural Resolution of Membrane Proteins: From Expression to Sample Preparation

This is the 1st Workshop from LINXS Membrane Protein Working Group
 

AIM

The aim is to provide expert overview about different platforms for
recombinant protein productions and purification strategies for
crystallography techniques. Overcoming low yield expression, misfolded
proteins and difficulties in purifying stable functional proteins are among
the most common bottlenecks delaying structural characterization. We invite
scientists to introduce their best practices in these subjects in order to
provide greater knowledge and knowledge transfer on how best to deal with
these challenges.
 

CONTRIBUTED TALKS

We hope to engage the attendance from PhD students and postdocs from a
range of disciplines, as well as more senior researchers. We are
encouraging participants, and especially PhD students, to consider
presenting their research in a contributed talk (call for abstracts) and
also to promote lively discussions.

CONTENT

*Day 1:*

 - Workshop aims and scope of the general problem
 - Membrane Protein Expression
 - Comparison of different expression systems
 - Success stories vs trouble-shooting problem areas, trial and error

 - Confirmed speakers:
 *Roslyn Bill* (Aston University) - *Recombinant membrane protein
 production in microbial hosts*
 *David Drew* (Stockholm University) - TBA
 *Joseph Lyons *(Aarhus University) - TBA

*Day 2:*

 - Sample Preparation, Quality Control
 - Membrane solubilization and other preparation methods

Confirmed speakers:
*Arjan Snijder* (AstraZeneca) - *Membrane protein purification and
application in drug discovery*
*Christopher G. Tate* (Cambridge University) - *Whipping GPCRs into shape
for structure determination*

In the autumn the plan is to continue further on topics about membrane
protein crystallization techniques, Cryo-EM or neutron structures,
scattering techniques and well-diffracting crystals.

Please find *Registration* and Timetable (will be updated with abstracts)
here: https://indico.linxs.lu.se/event/219/