3D solutions in Cryo-Electron Microscopy 2015
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In Maastricht
Objective
The aim of this workshop is to show, through lectures and practical courses, the solutions for 3D reconstruction in Cryo-Electron Microscopy.
Methodology
The lectures will cover single particle analysis, cryo-tomography of isolated specimens and cryo-tomography of structures inside a vitreous cryo-section or lamella and cryo-CLEM. The practical courses will show to the participants how to do sample preparation (high pressure freezing, plunge freezing, vitreous sectioning and cryo-fibbing), image acquisition in cryo-EM single-particle and cryo-tomography and 3D-reconstruction.
The aim of this workshop is to show, through lectures and practical courses, the solutions for 3D reconstruction in Cryo-Electron Microscopy.
Methodology
The lectures will cover single particle analysis, cryo-tomography of isolated specimens and cryo-tomography of structures inside a vitreous cryo-section or lamella and cryo-CLEM. The practical courses will show to the participants how to do sample preparation (high pressure freezing, plunge freezing, vitreous sectioning and cryo-fibbing), image acquisition in cryo-EM single-particle and cryo-tomography and 3D-reconstruction.
Image
