Fall 2024 Tomography and Cryo-FIB Workshop
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In Madison, WI
Topics include:
Introduction to cryo-ET including the basic principles of dose tolerance, illumination, and tilt series collection parameters.
Training in data acquisition workflows that include screening, data collection, and flexible scripting.
Training in basic data processing including pre-processing, tomogram generation, and considerations for segmentation and sub-tomogram averaging.
Instruction in 2D/3D correlative imaging workflows using the Leica widefield and cryo-confocal microscopes, and the integrated fluorescence microscope in the Aquilos 2.
Guidance in FIB-milling with AutoTEM and advanced FIB workflows such as waffle milling and lift-out methods.
Introduction to cryo-ET including the basic principles of dose tolerance, illumination, and tilt series collection parameters.
Training in data acquisition workflows that include screening, data collection, and flexible scripting.
Training in basic data processing including pre-processing, tomogram generation, and considerations for segmentation and sub-tomogram averaging.
Instruction in 2D/3D correlative imaging workflows using the Leica widefield and cryo-confocal microscopes, and the integrated fluorescence microscope in the Aquilos 2.
Guidance in FIB-milling with AutoTEM and advanced FIB workflows such as waffle milling and lift-out methods.
Contact:
Corina Elizabeth Evans