We are pleased to announce the Cryo-EM Workshop of the High-Resolution TEM
Winter School, which will be held on January 3-5, 2018 at Arizona State
University (ASU) Tempe campus. The HRTEM Winter School at ASU has a long
history in educating and training TEM scientists, and we have been
expanding our interests and courses to the biological applications,
especially the cryo-EM methods. Last year was our first annual cryo-EM
workshop, which brought success in promoting biological cryo-EM and
training more researchers into the field.

The goal of the Cryo-EM Workshop is to introduce the concepts and
applications of the biological cryo-EM. We will have two guest speakers
coming to join our workshop: Dr. Nikolaus Grigorieff (Howard Hughes Medical
Institute) and Dr. James Evans (Pacific Northwest National Laboratory).
Participants will learn the complete workflow and advanced techniques used
for biological cryo-EM through lectures, demos, and hands-on experiments.
No previous experience is required. Also, it will be a great opportunity
for the participants to learn the cryo-EM principles while working along
with their samples. Therefore, this year we are welcoming to receive the
proposals from who are interested in bringing their samples and processing
their data on site.

We are pleased to announce the 3rd International Conference on Three-dimensional Cryo-EM Image Analysis to be held March 21-24, 2018 at Granlibakken Conference Center, Lake Tahoe, California. The 2018 Symposium builds on our very successful previous meetings on the same theme in 2014 and 2016.

The goal of this Symposium Series is to discuss state of the art image analysis approaches and algorithm developments to tackle challenging biological problems and to identify current limitations in the field. We have recruited an outstanding panel of speakers.Â

The topics for this year's symposium are:

• Image Correction
• Reconstruction Methods
• Deep Learning/CNN Methods
• Automated Acquisition/Preprocessing
• Improving Maps
• Computing Hardware (GPU/Phi/Cloud/new/CPUs/storage)
• Selected Poster Talks

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A two day workshop on the subtomogram averaging software Dynamo led by the developer Daniel Castaño-Díez (University of Basel).Â

The workshop will provide hands-on training on all practical aspects of subtomogram averaging, encompassing all stages of the pipeline: tomogram visualization and archiving, particle picking techniques for different geometries, averaging, refinement and classification. No previous experience is required, and participants are welcome and encouraged to bring their own data sets.

The third SPHIRE workshopis to be held at Rice University Houston, TX. The workshop is free, but limited to 35 participants.
The target audience includes graduate students, postdocs, faculty and staff. This workshop will provide intensive hands-on-training in all practical aspects of single particle cryo-EM image processing using SPHIRE.
All stages of the SPHIRE pipeline will be covered, including:

• Micrograph movie alignment
• CTF estimation
• 2D classification
• 3D initial model generation
• High-resolution 3D refinement
• 3D focused classification and heterogeneity analysis

On July 6th and 7th, the European Photon & Neutron Campus in Grenoble will host a unique CRYO-EM Symposium in the framework of the newly established cryo-EM platform. The new facility will be open to the user community based on peer-review of scientific merit and technical feasibility. Scientists from ESRF, IBS and EMBL will support user operation.

Registration for the event is now closed, but you can follow the talks,
presented by worldwide experts in the field, through YouTube live streaming

https://www.youtube.com/user/LightforScience

The event will be live from 1.30pm on 6 July 2017.

Plenary Speakers

Allen Orville - Diamond Light Source Ltd

"Dynamic structural biology at XFELs with less sample, over longer reactions, and including complementary methods"

Neil Ranson - University of Leeds

"Cryo-EM as a tool in modern biomedical research"

The workshop will be held at the Research Center Caesar in the framework of the 6th International Caesar Conference: Overcoming barriers - Atomic-resolution and beyond: advances in molecular electron microscopy (12-15th Sept. 2017).

The workshop is exclusive to registered participants of the conference, but provided at no additional costs. The target audience includes graduate students, faculty and staff.

This workshop will provide intensive hands-on-training in all practical aspects of single particle cryo-EM image processing using SPHIRE.

All stages of the SPHIRE pipeline will be covered, including:

• Micrograph movie alignment
• CTF estimation
• 2D classification
• 3D starting model generation
• High-resolution 3D refinement
• 3D focused classification and heterogeneity analysis

Registrations to the workshop will be accepted on a first-come-first-serve basis, however limiting participants to one per group/department. Confirmation letters will be sent latest beginning of August.
The workshop is limited to 20 attendees. Computing hardware will be provided in place.
You can find more information and register at:
https://www.caesar.de/en/events/2017/6th-international-caesar-conference-overcoming-barriers.html
Please remember to use the subject "Workshop SPHIRE Registration" when registering for the workshop, as there will also be a parallel workshop on sample preparation and tomography.

This will be a hands-on workshop on the new software tool Fluctuating Finite Element Analysis (FFEA), which calculates dynamic trajectories for flexible biomolecules directly from cryo-EM/ET density maps. FFEA aims to provide an equivalent simulation tool for the EMDB as atomistic MD simulations have for the PDB. This approach uniquely uses volumetric meshes rather than atomistic co-ordinates as input such that it can model protein dynamics, protein-protein interactions, conformational switching between known configurational states and can be mapped back to atomistic co-ordinates, where these are available. This workshop is ideal for biomolecular simulators who would like to make full use of cryo-EM data in their modelling, or for experimentalists who would like to gain new theoretical insight into the dynamic behaviour of the structures they observe.

Registration: https://eventbooking.stfc.ac.uk/news-events/ffea-practical-workshop-381

From Structure and Dynamics to Energy Landscapes and Personalized Medicine

Session I â€" Overview and principles

An overview of cryoEM: Richard Henderson (LMB-MRC, Cambridge, UK)

A Bayesian view on cryo-EM structure determination: Sjors Scheres (LMB-MRC, Cambridge, UK)

From hybrid methods to direct structure solving by cryoEM: a Spanish perspective: Jose López Carrascosa (CNB-CSIC, Madrid)

How to overcome biochemical limitations in sample preparation for high-resolution structure determination of macromolecular complexes by cryo-EM and X-ray crystallography: Holger Stark (MPIBPC, Göttingen, Germany)

Session II â€" Solution and reconstruction

EM reconstruction from the standpoint of Maximum Entropy estimation: Jorge Navaza (CNRS Emeritus at the École Normale Supériere de Cachan)

Solving X-ray crystal structures with cryo-EM reconstructions: Randy Read (CIMR, University of Cambridge, UK)

Repurposing molecular replacement algorithms for docking models in Cryo-EM reconstructions: Airlie McCoy (CIMR, University of Cambridge, UK)

Model-building in Low Resolution and Cryo-EM maps using Coot: Paul Emsley

Session III â€" Model Building to automation

Automated model building in cryoEM maps: Jon Agirre (YSBL, University of York, UK)

New approaches to the analysis of cryoEM maps quality:José María Carazo (CNB-CSIC, Madrid)

Structural studies of heterogeneous assemblies: Elena Orlova

Model-building using cryo-EM and crystallographic maps: Tom Terwilliger (Los Alamos National Laboratory, USA

Session IV â€" Refinement and Global Initiatives

 Refinement of atomic models into cryo-EM maps using REFMAC5: Garib Murshudov (LMC-MRC, Cambridge, UK).

New real space refinement methods for cryo-EM: Pavel Afonine (Lawrence Berkeley National Laboratory, Berkeley CA, USA)

The intersection of crystallography and cryo-EM in Phenix, new tools for both: Paul Adams (Lawrence Berkeley National Laboratory, Berkeley CA, USA)

Introducing the Collaborative Computational Project for Electron cryo-Microscopy: CCP-EM: Tom Burnley (CCPEM, UK)

Session V â€" Application and Future prospects

Investigating membrane protein structure and function by cryo-EM: Werner Kühlbrandt (Max-Plank Institut für Biophysik)

The emerging role of CryoEM in drug discovery and development: Paula da Fonseca (LMB-MRC, UK)

Cryo-EM at near-atomic resolution of icosahedral viruses: José Castón (CNB-CSIC, Madrid)

CryoEM illuminates the world of the Picornaviruses: Abhay Kotecha (Division of Structural biology, University of Oxford, UK)

Mechanistic insights on DNA replication and repair by cryo-EM: Rafael Fernandez Leiro (LMB-MRC, UK)

Structural studies of functional ribosome complexes: Paola Fucini (CIC-Biogune, Bilbao)

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The 6^th International caesar Conference presents leading research in molecular electron microscopy of dynamic biological entities.

The conference focuses on recent technological as well as methodological advances and their applications to fundamental biological questions.

Prior to the conference, there is a workshop (Monday & Tuesday, Sept. 11-12) on molecular Cryo-EM. The workshop covers both tomographic as well as single-particle techniques and is free of charge for registered conference participants. It aims at graduate students and (junior) postdocs. The workshop is limited to 20 participants. To register for the workshop, send an email to [email protected] using the subject "Workshop Registration".