Applications are now open for the Instruct Theoretical and Practical Course: Integrative Structural Biology in Latin America, from 23 â€" 27 November 2020. This remote course, which is open to Latin American graduate students and postdocs, will cover a broad range of structural biology techniques, including X-ray crystallography, X-ray scattering, NMR and electron microscopy, with a focus on current strategies used to integrate the information gathered from different techniques.

To be presented from the Center for BioMolecular Structures, Brookhaven National Laboratory.
LIX (morning) (Event ID:  E000003456) and Macromolecular Crystallography and MX (Event ID:  E000003457)(afternoon) Workbenches
The main goal of these VIRTUAL workshops is to provide an opportunity to get acquainted with the method and software utilized for data collection. Lectures and demonstrations will address the capabilities of the beam lines and provide an overview of Structural Biology resources available to the community at NSLS II. Tips and Tricks on how to collect the best data will be discussed throughout the workshops. We encourage you to send samples and take advantage of the time reserved for the workbench to analyze a few samples.

REGISTRATION Deadline: October 23, 2020
https://www.bnl.gov/lixworkbench/
 

https://www.bnl.gov/mxworkbench/
 

Please do register for both workshops separately if you plan to participate in both.

7th joint Diamond-CCP4 Workshop

 Bringing together leading experts in the field of MX to teach best
practice in data collection and analysis, this course is aimed at PhD
students, postdocs and early career scientists who have a focus on
structural biology.

The workshop will take place online via Zoom over the course of two
full weeks, as well as a preparation day in advance of the workshop.
It is essential that applicants commit to attending the workshop in
its entirety. Please note below the workshop days and timings
involved:

- Preparation day: Monday 23 November 2020
- Week 1: Monday 30 November to Friday 4 December 2020
- Week 2: Monday 7 December to Friday 11 December 2020
Each day will begin at 08:45 and will finish at 17:15 (GMT - UK Time)

Some prior experience of crystallography and data collection is
expected, and those who can bring an interesting project with them
(crystals and possibly previously collected datasets) will be given
priority.

There is no fee to attend this online workshop, however attendance
will be subject to an application process and a letter of support from
the attendee's supervisor will be required. Both the application form
and supervisor's letter of support will need to be submitted by 17:00
(GMT) on Friday 16 October 2020.

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This conference will bring together experts and early stage researchers from the separate fields

• to provide a platform for structural biology within the cellular context
• to stimulate discussions about how to meaningfully integrate data from different methods
• to define what types of biological questions can already be addressed using in situ structural biology and which might be approached in the future

Session Topics

• Imaging across scales
• Structural analysis in situ
• Molecular sociology
• Biophysical analysis in cells
• Integrative modelling

Amsterdam Scientific Instruments is hosting an online seminar by Mauro Gemmi, PhD, director from Center for Nanotechnology Innovation@NEST on the topic of “3D electron diffraction: from inorganic nanoparticles to protein nanocrystals”. It will take place in two time slots on the 30th of June, 8.30 am and 5 pm CET time.

With the following link you can register for the online seminar on the

30th of June at 8.30 am: https://cutt.ly/zujlH27 (Password: cpEZHWk6f67

(27394956 from phones and video systems))

The following link allows you registration on the second time slot on

the

30th of June, at 5 pm: https://cutt.ly/9ujzi4i

(Password: C2MgM2dCcf6 (22646232 from phones and video systems)

On Wednesday 8 July 2020

Session 1: 9:00 am CEST (Berlin) and 3:00 pm SGT (Singapore)

Session 2: 6:00 pm CEST (Berlin) and 11:00 am CDT (Chicago)

Speakers: Dr Vernon Smith and Dr Martin Adam

On Tuesday 23 June 2020: 9:00 am CDT (Chicago) and 4:00 pm CEST (Berlin)

Speakers: Dr Arnaud Basle and Dr Michael Mrosek

On Tuesday 16 June 2020: 10:00 am CDT (Chicago) and 5.00 pm CET (Berlin)

Speakers:Â Dr Michael Ruf and Dr Jurgen Graf

The NSLS-II (MX beamlines) is organizing a workshop entitled "Measuring Protein Dynamics using Very High Throughput Automated Micro-beam and Time Resolved Micro-beam Macromolecular Crystallography".

The format of the workshop is online (using BlueJeans and is scheduled to take place on May 18 and May 19 from 1 PM (ET) to 5.30 PM (ET) both days.

There is no fee to participate in the workshop.

Monday Session: Protein Dynamics from large number of structures.

Confirmed speakers and titles:
Yingkai Zhang (NYU): "Ab initio QM/MM Molecular Dynamics Simulations of Enzyme Reactions"

Rebecca Page (University of Arizona): " Title: The dynamics of phosphorylation signaling"

Dmytro Kozakov (Stony Brook University): "TBA"

Gino Cingolani (Thomas Jefferson University): "TBA"

Thu NGuyen (Stony Brook University): "Computational methods to discover small differences in protein structures"

Tuesday Session: Time Resolved MX.

Confirmed speakers and titles:
Pedram Mehrabi (Max Planck Institute): "Time-resolved serial synchrotron crystallography for the functional characterization of proteins"

Diana Monteiro (Hauptman-Woodward Medical Research Institute): Protein triggering and sample delivery for time-resolved diffraction and scattering experiments using synchrotron sources

Jonathan Clinger (Cornell University): "Development of an Alternative Approach to Time-Resolved X-ray Crystallography"

Zhong Ren (University of Illinois at Chicago): "In Situ Serial Crystallography at Room Temperature"

On Wednesday 13th May 2020: 10:00am CDT (Chicago) and 5.00pm CET (Berlin)

Speakers: Dr Matthew Benning and Dr Vernon Smith

The workshop has been designed for postgraduate, postdoctoral and research scientists that deal with macromolecular crystallography and cryo EM but need further knowledge on the routine uses, the state of the art and fundamentals that underlie the modern techniques for macromolecular crystallography and cryo EM. The topics will cover crystallographic computing and fundamentals using XDS, CCP4 and Phenix, as well as the state of the art on topics such as the strategies for protein production, biophysical methods, data collection, phasing, automated pipelines at synchrotron and refinement with ARCIMBOLDO, SHELX and SIR2014 and modeling with COOT.

MCS2020 will provide six days of lectures and workshops. The course is designed for 25 highly motivated attendants.


Requests to participate must be sent by a free-formated E-mail, before March 11th, 2020 to the following address: [email protected] , indicating the motivation to participate and experience on macromolecular crystallography and including a letter of recommendation from PhD Director (only for PhD students).

Topics

• Sample preparation
• Structural and functional characterization
• Computational analysis of integral membrane proteins

The workshop includes a symposium, lectures, and hands-on experiments at the High Flux Isotope Reactor (IMAGINE, BIO-SANS) and the Spallation Neutron Source (EQ-SANS, LIQREF, MaNDi, Neutron Spin Echo Spectrometer). The workshop is designed for graduate students, post-doctoral researchers and faculty with knowledge of structure and function of biological macromolecules but with limited to no experience with neutron sciences.

Satellite Workshop of the XXV IUCr Congress

"Macromolecular crystallography (MX) is the dominant means of determining the three-dimensional structures of biological macromolecules. Over the last few decades, most MX data have been collected at synchrotron beamlines using a large number of different detectors produced by various manufacturers and taking advantage of various protocols and goniometry. These data came in their own formats, sometimes proprietary, sometimes open. The associated metadata rarely reached the degree of completeness required for data management according to Findability, Accessibility, Interoperability and Reusability (FAIR) principles. Efforts to reuse old data by other investigators or even by the original investigators some time later were often frustrated. In the culmination of an effort dating back more than two decades, a large portion of the research community concerned with High Data-Rate Macromolecular Crystallography (HDRMX) has now agreed to an updated specification of data and metadata for diffraction images produced at synchrotron light sources and X-ray free electron lasers (XFELs). This Gold Standard will facilitate processing of datasets independent of the facility at which they were collected and enable data archiving according to FAIR principles, with a particular focus on interoperability and reusability. This agreed standard builds on the NeXus/HDF5 NXmx application definition and the International Union of Crystallography (IUCr) imgCIF/CBF dictionary and is compatible with major data processing programs and pipelines. Just as with the IUCr CBF/imgCIF standard from which it arose and to which it is tied, the NeXus/HDF5 NXmx Gold Standard application definition is intended to be applicable to all detectors used for crystallography, and all hardware and software developers in the field are encouraged to adopt and contribute to the standard." [A. Förster, H. J. Bernstein, A. Bhoemick, A. S. Brewster, S. Brockhauser, L. Geliso, D. R. Hall, F. Leonarski, V. Mariani, G. Santoni, C. Vonrhein, G. Winter, "A Gold Standard for Macromolecular Diffraction Data", in preparation]

This is a tutorial workshop presented by the developers of the Gold Standard to introduce the community to this important upgrade to the interoperability and reusability of macromolecular crystallographic data for both synchrotrons and XFELS,and to give the participants an opportunity to work with and comment on the Gold Standard. To widely share best practices for metadata recording, we encourage the participation of neutron and chemical crystallographers.

The workshop will function as a purely virtual meeting via Zoom from 9 am to 3 pm Prague time (3 am to 9 pm New York time, 8 am to 2 pm London time) on 14 August 2021 with two breaks. In order to participate and receive the Zoom meeting room URL and agenda, contact Herbert J. Bernstein, [email protected], giving your name, institutional affiliation and email address by no later than 6 pm Prague time (noon New York time, 5 pm London time) on Friday 6 August 2021. Registration is limited to 100 persons. There will be no additional fee for the workshop virtual meeting registration.

Reminder: The CommDat user forum is at https://forums.iucr.org

• data processing;
• phasing and structure determination;
• model refinement and validation;
• introduction to crystallography + cryo-electron microscopy integration