Crystal structure of Mycobacterium tuberculosis mtFabD, a malonyl-CoA:ACP transacylase (MCAT)
Mycolic acids - very long α-alkyl, β-hydroxylated fatty acids - are essential and characteristic components of the cell envelope of Mycobacterium tuberculosis
, and are synthesised by the joint action of the type I and II fatty acid synthase (FAS) systems. The 3.0 Å crystal structure of M. tuberculosis
FabD, which catalyses acyl transfer from coenzyme A to acyl carrier protein of FAS-II, was phased based on anomalous scattering of five ordered Ni2+
ions bound to the His6
-affinity tag of the recombinant enzyme.
H. Ghadbane, A.K. Brown, L. Kremer, G.S. Besra and K. Fütterer