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Workshop on small-angle x-ray scattering/diffraction in biology

  • Subject: Workshop on small-angle x-ray scattering/diffraction in biology
  • From: tsuruta@SLAC.Stanford.EDU
  • Date: Wed, 30 Sep 1998 14:56:23 -0700 (PDT)

Workshop on Small-angle X-ray Scattering/Diffraction in Biology
October 21, 1998.

Stanford Synchrotron Radiation Laboratory
Stanford Linear Accelerator Center

Please check out http://www-ssrl.slac.stanford.edu/ssrl25/index.html for 
registration information.  The workshop is held in association with the 
25th SSRL users' conference, October 19-20.


This workshop is designed to give an overview of solution x-ray 
scattering and low-angle single-crystal diffraction in structural 
molecular biology.  It consists of lectures on recent developments 
in the field, software demonstrations and hands-on sessions on data 

Topics in solution scattering include: determining low-resolution 
structures, modeling quaternary structure, complementing atomic-resolution 
structures, time-resolved studies and use of x-ray scattering amplitudes 
in electron microscopy and diffraction.  Hands-on sessions and software 
demonstrations are intended for scientists who are new to the technique.

SSRL has developed an instrument which makes it practical to obtain 
accurately measured single crystal diffraction data in the range of 
10-500 A, where the contribution of the molecular envelope is much 
greater than at higher resolution.  The diffraction data in this 
resolution range are thus critical in phase extension and density 
averaging.  The data have the potential of visualizing parts of 
structure that are otherwise averaged out to very weak density.  
Technical issues in dealing with very low resolution data will be 

Preliminary program

 9:00 Introduction on small-angle scattering/diffraction
      - Hiro Tsuruta (SSRL)
 9:20 Computational techniques for solution scattering data 
      - Dmitri Svergun (European Molecular Biology Laboratory)
10:00 Coordinated use of solution x-ray scattering and electron      
      cryomicroscopy of macromolecules
      - Wah Chiu (Baylor College of Medicine)
10:30 coffee break
10:45 Imaging viral capsids and RNA with low angle solution and single 
      crystal diffraction data.
      - John E. Johnson (The Scripps Research Institute)
11:15 Modeling viruses and processing data with icosahedral harmonics. 
      - Peter Doerschuk (Purdue University)
11:45 The use of low resolution data in imaging the nucleic acids in 
      icosahedral viruses.
      - Vijay S. Reddy (The Scripps Research Institute)
12:15 lunch break
13:15 (Phase extension and density averaging using low-res. diffraction 
      data in crystallographic studies on DNA helicase)
      - Christpher Putnam (The Scripps Research Institute)
13:45 Measurements and analysis of time resolved SAXS for protein folding
      - Sebastian Doniach (Stanford University)
14:15 A computer system for solution scattering data analysis. [computer 
      software demonstrations]
      - Dmitri Svergun (EMBL)
15:00 coffee break
15:15 Data collection practice on Beam Line 4-2 [hands-on sessions]
      1) solution scattering
      2) low-angle single crystal diffraction