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new method

Dear Colleagues:

I am posting  this message to get your assistance or advice. I would love 
to continue the study of  plasma lipoproteins by small-angle X-ray scattering 
( SAXS )and complete the development of new noninvasive screening 
method to detect a coronary atherosclerosis  in its earliest stages. 

During the last several years I have been researching on the plasma 
lipoproteins from human blood by SAXS. As a result, the rapid (within 1 h)
technique to determine the fraction distribution of plasma lipoproteins has
been developed. A new effective compound like NaBr for "X-ray visualizing" 
the lipid cores of lipoprotein particles into the complex lipid-protein mixture 
of human plasma has been found. In contrast to conventional procedures for 
quantitative determination of plasma lipoprotein fractions, the new method 
allows one to "fractionate" the total plasma lipoproteins into three major 
classes such as high density, low density, and very low density lipoproteins 
without a preliminary separation of human plasma into its constituents. The 
findings demonstrate the diagnostic application  of SAXS in atherosclerosis.

At the present time I am living in the United States and looking for the 
opportunities for applying SAXS as the analytical instrument for coronary 
atherosclerosis susceptibility express-diagnosis (see attachment). 

I hope my proposal will find your interest. I am looking forward to receiving 
your advice and assistance. Thanks in advance. 


Vladimir Vavilin, Ph.D.

108 Enchanted Hills Rd., Apt.104                Phone: 410-581-1023
Owings Mills, MD 21117                            E-mail: viv@erols.com  

       Coronary heart disease is the most frequent cause of death in men over 
age 35 and women over age 65 in the United States. For example, in 1987 
estimated 514,000 Americans were killed by this disease and over  700,000 
others were admitted to hospitals because of heart attacks. 
       Coronary heart disease results from the gradual development of 
atherosclerosis in the coronary arteries. Therefore, it is very important 
to predict the human susceptibility to coronary atherosclerosis in its earliest
       High density lipoproteins (HDL) and low density lipoproteins (LDL) 
fulfilling  central functions in   the cholesterol transport process by the blood 
circulation are known to play the key roles in the atherosclerotic pathogenesis. 
It is also known that high concentrations of LDL as well as low concentrations 
of HDL in the human bloodstream are associated with the accelerated 
progression of the coronary atherosclerosis. Hence it follows an important 
conclusion that it is necessary to measure the absolute concentrations of these 
two major lipoprotein classes for diagnosing the susceptibility to 
       In this connection new method to diagnose the susceptibility to 
atherosclerosis in humans have been developed. The devised method is 
based on the analysis of experimental data obtained by means of small-angle 
X-ray scattering technique which enables one to determine a particle size 
distribution of lipid cores of major plasma lipoproteins (HDL, LDL and 
very low density lipoproteins) and  evaluated an index of susceptibility to 
atherosclerosis (LDL/HDL ratio).
       This method has the considerable advantages in comparison with the 
conventional procedures for the quantitative determination of the human 
plasma lipoprotein fractions. It has following advantages: just several drops
(0.1mL) of a human plasma need for one analysis that lasts only 60 min 
and it does not demand a preliminary separation of a human  plasma into 
its constituents. The latter it is very important because the human plasma 
lipoproteins are labile structures and therefore the isolated lipoproteins 
can be differed from the native lipoproteins after separation and storage 
       In conclusion one should note that the proposed method allows one 
to create quite new analytical instrument for early express-diagnosis of the 
human susceptibility to coronary atherosclerosis and carry on a mass health 
screening of the American people for detecting the individuals with high 
risk of coronary heart disease.