IUCr journals
[Schematic design]Top left - Schematic design of goniometer set-up; top right - crystals and beam relationship; bottom left - xyanalase II structure; bottom right - 2Fo-Fc density map

Protein crystallography with a micrometre-sized synchrotron-radiation beam

Acta Cryst. (2008). D64, 158-166 [doi:10.1107/S090744490705812X]

Recent advances in instrumentation at the microfocus beamline (ID13) at the European Synchrotron Radiation Facility allowed a protein microcrystallography experiment with a focused synchrotron-radiation beam of 1 micron. High-resolution diffraction patterns of xylanase II were obtained from 20 μm3 crystal volumes with a flux density of 3 × 1010 photons/sec/μm2 at the sample. In spite of the high irradiation dose, a 1.5 Å resolution map for a xylanase II structure was obtained with no evidence of radiation damage. This result opens many new opportunities in protein microcrystallography.

R. Moukhametzianov, M. Burghammer, P.C. Edwards, S. Petitdemange, D. Popov, M. Fransen, G. McMullan, G.F.X. Schertler and C. Riekel