IUCr journals

Rv0802c from Mycobacterium tuberculosis: the first structure of a succinyltransferase with the GNAT fold

Acta Cryst. (2008). F64, 978-985 (doi.org/10.1107/S1744309108031679)

[Electron density map for acetate] Electron density (Fo - Fc OMIT map at 4σ) for a bound acetate ion discovered serendipitously within the active site of 'unliganded' Rv0802c.
GCN5-related N-acetyltransferases (GNATs) typically transfer an acetyl group from AcCoA to a diverse set of amine-containing substrates. The structure of Rv0802c, a hypothetical GNAT from Mycobacterium tuberculosis, was determined and found to contain a multicoordinated acetate ion. The location of the acetate ion suggested an alternative acyl donor for Rv0802c, which was borne out by the structure determination of Rv0802c with bound succinylCoA. Structural analysis of Rv0802c was therefore crucial in revealing that a subset of GNAT family members can utilize succinylCoA as a cofactor.

M.W. Vetting, J.C. Errey and J.S. Blanchard