Expression, purification and X-ray crystallographic analysis of thioredoxin from Streptomyces coelicolor
Acta Cryst. (2005). F61, 164–168
The Gram-positive bacterium
Streptomyces coelicolor contains three thioredoxins that are involved in unknown biological processes.
trxA from
S. coelicolor was cloned and expressed in
Escherichia coli and the protein purified and crystallized using the hanging-drop method of vapour diffusion. The crystal structure of thioredoxin A has been determined at 1.5 Å resolution using synchrotron radiation. The crystal exhibits the symmetry of space group
P2
12
12, with unit-cell parameters
a = 43.6,
b = 71.8 and
c = 33.2 Å.
Petra Stefankova, Jana Maderova, Imrich Barak, Marta Kollarova and Zbyszek Otwinowski