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Expression, purification and X-ray crystallographic analysis of thioredoxin from Streptomyces coelicolor

Acta Cryst. (2005). F61, 164–168

[Streptomyces coelicolor] Overall structure of the TrxA molecule from S. coelicolor. α-Helices are shown in purple and β-strands in yellow.
The Gram-positive bacterium Streptomyces coelicolor contains three thioredoxins that are involved in unknown biological processes. trxA from S. coelicolor was cloned and expressed in Escherichia coli and the protein purified and crystallized using the hanging-drop method of vapour diffusion. The crystal structure of thioredoxin A has been determined at 1.5 Å resolution using synchrotron radiation. The crystal exhibits the symmetry of space group P21212, with unit-cell parameters a = 43.6, b = 71.8 and c = 33.2 Å.
Petra Stefankova, Jana Maderova, Imrich Barak, Marta Kollarova and Zbyszek Otwinowski