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Low-resolution structure determination of Na+-translocating NADH: ubiquinone oxidoreductase from Vibrio cholerae by ab initio phasing and electron microscopy

Acta Cryst. (2012). D68, 724-731 (

[Na-NQR] Overlay of the 16 Å crystal structure (yellow) and the EM reconstruction (grey). (a) Side view, (b) top view of the Na+-NQR complex.

A 16 Å resolution envelope of the membrane protein Na+-NQR was determined by connectivity-based ab initio phasing. The found envelope was used as a reference in three-dimensional reconstruction of the Na+-NQR from negative-stain EM images. The two methods displayed similar dimensions and features of the particle. Despite the resolution limitations, the method could provide important structural information in many X-ray crystallographic projects where the molecule of interest is a membrane protein or membrane protein complex yielding crystals of low diffractive power.

V. Y. Lunin, N. L. Lunina, M. S. Casutt, K. Knoops, C. Schaffitzel, J. Steuber, G. Fritz and M. W. Baumstark