Protein Data Bank exchange dictionary (pdbx) version 1.0521

Category PDBX_ENTITY_SRC_GEN_PROD_PCR

Name:
'pdbx_entity_src_gen_prod_pcr'

Description:

   This category contains details for the PCR steps used in
   the overall protein production process. The PCR is assumed
   to be applied to the result of the previous production step, or the
   gene source if this is the first production step.

Example:

Example 1 - hypothetical example

 
    _pdbx_entity_src_gen_prod_pcr.entry_id           111000111
    _pdbx_entity_src_gen_prod_pcr.entity_id          222000111
    _pdbx_entity_src_gen_prod_pcr.step_id            1
    _pdbx_entity_src_gen_prod_pcr.next_step_id       2
    _pdbx_entity_src_gen_prod_pcr.end_construct_id   440050000111
    _pdbx_entity_src_gen_prod_pcr.robot_id           5
    _pdbx_entity_src_gen_prod_pcr.date               '2002-07-12:15:13'
    _pdbx_entity_src_gen_prod_pcr.forward_primer_id  2
    _pdbx_entity_src_gen_prod_pcr.reverse_primer_id  3
    _pdbx_entity_src_gen_prod_pcr.reaction_details
  ;  Annealing temperature = 70 C
     Annealing time = 60 s
     Extending temperature = 74 C
     Extending time = 120 s
     Melting temperature = 95 C
     Melting time = 120 s
     Number of cycles = 40
     Polymerase = KOD
     Template = 10 pmol
     Primer = 25 pmol
     Total volume = 25 ul
  ;
    _pdbx_entity_src_gen_prod_pcr.purification_details
  ;  No purification
  ;

Category groups:
    inclusive_group
    protein_production_group
    entity_group
    pdbx_group
Category keys:
    _pdbx_entity_src_gen_prod_pcr.entry_id
    _pdbx_entity_src_gen_prod_pcr.entity_id
    _pdbx_entity_src_gen_prod_pcr.step_id

Mandatory category: no

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Crystallographic Information Framework

Protein Data Bank exchange dictionary (pdbx) version 1.0521

Category PDBX_ENTITY_SRC_GEN_PROD_PCR

International Tables for Crystallography

Volume G: Definition and exchange of crystallographic data

Edited by S. R. Hall and B. McMahon

IUCrData